The aim of this work was to optimize a maceration condition of cannabis (Cannabis sativa L.). A circumscribed central composite experimental design was applied in this work. Temperature and time were varied from 40-80 °C and 30-90 min, respectively. The three responses (i.e., extraction yield, cannabidiol content, and Δ9-tetrahydrocannabinol content) were predicted by computer software. The yield was high when cannabis was macerated using ethanol at high temperature and long duration time. While cannabidiol and Δ9-tetrahydrocannabinol content was high when macerating at a low heating temperature and short duration time. The optimal condition provided the simultaneous high of cannabidiol and Δ9-tetrahydrocannabinol content was 40 °C for 30 min. The prediction was accurate due to low percent error. This optimal condition could be used as a guide for maceration of cannabis to obtain the extract containing a high content of cannabidiol and Δ9-tetrahydrocannabinol.
Monton, C., Madaka, F., Settharaksa, S., Wunnakup, T., Suksaeree, J., & Songsak, T. (2019). Optimal condition of cannabis maceration to obtain the high cannabidiol and Δ9-tetrahydrocannabinol content. Anais Da Academia Brasileira de Ciencias, 91(3). https://doi.org/10.1590/0001-3765201920190676