In vitro cytotoxic activity of Isostichopus badionotus, A sea cucumber from Yucatan Peninsula coast

Abstract

The in vitro cytotoxic activity of hexane, ethyl acetate and butanol extracts of the sea-cucumber Isostichopus badionotus (Holothuroidea) was tested against normal cells (Vero), human cervical carcinoma (HeLa) and breast adenocarcinoma (MCF-7 and MDA-MB-231) ATCC cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Hexane extracts from body walls and viscera showed high cytotoxic activity against HeLa cells (IC 50 's = 48.5 and 42.5 μg mL -1, respectively), while the ethyl acetate extract of body walls was considered low active (IC 50 = 98.3 μg mL -1). In addition, the body walls hexane extract showed a good selectivity index value of 12.0. © 2014 Lifescience Global.