High-resolution NMR-based metabolic detection of microgram biopsies using a 1 mm HRμMAS probe

Abstract

A prototype 1 mm High-Resolution micro-Magic Angle Spinning (HRμMAS) probe is described. High quality 1H NMR spectra were obtained from 490 μg of heterogeneous biospecimens, offering a rich-metabolite profiling. The results demonstrate the potential of HRμMAS as a new NMR analytical tool in metabolomics.