Characterization of endophytic plant growth promoting traits of Methylobacterium sp. isolated from banana (Musa sp.)

Abstract

A total of 26 endophytic Methylobacterium sp. strains were obtained from surface sterilized leaves of two banana\rcultivar (Robusta and Nattu poovan) and the strains were tested for its ability to fix atmospheric nitrogen, EPS and IAA production\rfor promoting banana tree growth. The result of the present study demonstrated that endophytic population ranged from 4.03 and\r4.14 log cfu per gram of leaf tissue. Among these four isolates were chosen based on colony morphology and their distinct\rpigmentation. All the selected four isolates were able to grow in nitrogen free methanol mineral salt medium. The synthesis of\rindole-3-acetic acid (IAA) in the presence of L-tryptophan was detected in all the isolates tested. The isolate FM2\r(Methylobacterium sp.) produced the highest amount of IAA (13.01mgml-1) in medium supplemented with L-tryptophan and was\rable to synthesize IAA in the absence of L-tryptophan. The maximum amount of ASP and WSP was recorded in FM3 (21.73 μg\rml-1) and FM1 (157.79 μg ml-1), respectively. They were tentatively identified at species level based on carbon utilization test. The\rclassified strains were also screened for methanol dehydrogenase (mxaF gene sequencing) using specific primers and obtained\r555 bp PCR product. So the Methylobacterium sp. strains analyzed here had a promising potential for developing as a plant\rgrowth promoting bacteria for sustainable agriculture.