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Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples

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Abstract

On-chip detection of low abundant protein biomarkers is of interest to enable point-of-care diagnostics. Using a simple form of integration, we have realized an integrated microfluidic platform for the detection of prostate specific antigen (PSA), directly in anti-coagulated whole blood. We combine acoustophoresis-based separation of plasma from undiluted whole blood with a miniaturized immunoassay system in a polymer manifold, demonstrating improved assay speed on our Integrated Acoustic Immunoaffinity-capture (IAI) platform. The IAI platform separates plasma from undiluted whole blood by means of acoustophoresis and provides cell free plasma of clinical quality at a rate of 10 uL/min for an online immunoaffinity-capture of PSA on a porous silicon antibody microarray. The whole blood input (hematocrit 38-40%) rate was 50 μl min-1 giving a plasma volume fraction yield of ≈33%. PSA was immunoaffinity-captured directly from spiked female whole blood samples at clinically significant levels of 1.7-100 ng ml-1 within 15 min and was subsequently detected via fluorescence readout, showing a linear response over the entire range with a coefficient of variation of 13%. © 2013 The Royal Society of Chemistry.

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Tajudin, A. A., Petersson, K., Lenshof, A., Swärd-Nilsson, A. M., Åberg, L., Marko-Varga, G., … Laurell, T. (2013). Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples. Lab on a Chip, 13(9), 1790–1796. https://doi.org/10.1039/c3lc41269e

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