Contribution of soil microbial necromass carbon to soil organic carbon fractions and its influencing factors in different grassland types

Abstract

Microbial necromass carbon (MNC) is a significant source of soil organic carbon (SOC). However, the contribution of microbial necromass to different organic carbon fractions and their influencing factors in various soil layers under different grassland types remains unclear. This study was conducted through a comprehensive investigation of soil profiles (0–20, 20–40, and 40–100 cm) across four grassland types in Ningxia, China, encompassing meadow steppe, typical steppe, desert steppe, and steppe desert. We quantified mineral-associated organic carbon (MAOC), particulate organic carbon (POC), and their respective microbial necromass components, including total microbial necromass carbon (TNC), fungal necromass carbon (FNC), and bacterial necromass carbon (BNC), and analyzed the contributions to SOC fractions and influencing factors. Our findings reveal three key insights. First, the contents of MAOC and POC in the 0–100 cm soil layer were in the following order of magnitude: Meadow steppe > Typical steppe > Desert steppe > Steppe desert, with the average content of POC being 9.3 g kg−1, which was higher than the average content of MAOC (8.73 g kg−1). Second, the content of microbial TNC in MAOC and POC decreased with soil depth, the average content of FNC was 3.02 and 3.85 g kg−1, which were higher than the average content of BNC (1.64 and 2.08 g kg−1). FNC dominated both MAOC and POC, and its contribution was higher than the contribution of BNC. Third, through regression analysis and random forest modeling, we identified key environmental drivers of MNC dynamics: mean annual rainfall, electrical conductance, and soil total nitrogen emerged as primary regulators in surface soils (0–20 cm), while available potassium, SOC, and mean annual temperature dominated deeper soil layers (20–100 cm). This research contributes by: (1) establishing the vertical distribution patterns of MNC and SOC fractions in soil profiles; (2) quantifying the relative contributions of MNC to SOC fractions across different grassland ecosystems soil profiles and elucidating their environmental controls, offers a deeper understanding of the mechanisms driving MNC accumulation in SOC fractions in diverse grassland ecosystems, and providing data support for further research on the microbiological mechanisms of soil organic carbon formation and accumulation in arid and semi-arid regions.