Aproximación a una caracterización molecular de Fasciola hepatica por la técnica RAPDs - PCR

Abstract

The present study show the molecular characterization of Fasciola hepatica taken from cows, horses and sheeps, using the Random Amplified Polymorphic ADN Fragments (RAPDs-PCR) technique. The standardization of the optimal conditions of amplification and thermocyclation for F. hepatica by RAPDs-PCR were made, as the genetic markers for polymorphic identification of the parasites collected from different animals specie. The methodology used compared the genetic pattern between species and inside each specie. The results shows random genetic markers, given genetic variations of F. hepatica between species and inside each specie (polymorphism), and the amplifications fragments were between 135 and 741 pair of bases (bp).