The Influenza Virus Protein PB1-F2 Interacts with IKKβ and Modulates NF-κB Signalling

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Abstract

PB1-F2, a protein encoded by a second open reading frame of the influenza virus RNA segment 2, has emerged as a modulator of lung inflammatory responses but the molecular mechanisms underlying this are only poorly understood. Here we show that PB1-F2 inhibits the activation of NF-κB dependent signalling pathways in luciferase reporter assays. PB1-F2 proteins from four different viruses interact with IKKβ in yeast two-hybrid assays and by co-immunoprecipitation. PB1-F2 expression did not inhibit IKKβ kinase activity or NF-κB translocation into the nucleus, but NF-κB binding to DNA was severely impaired in PB1-F2 transfected cells as assessed by Electrophoretic Mobility Shift Assay. Neither the N-terminal 57 amino acid truncated forms nor the C-terminus of PB1-F2 were able to inhibit NF-κB dependent signalling, indicating that the full length protein is necessary for the inhibition. © 2013 Reis, McCauley.

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Reis, A. L., & McCauley, J. W. (2013). The Influenza Virus Protein PB1-F2 Interacts with IKKβ and Modulates NF-κB Signalling. PLoS ONE, 8(5). https://doi.org/10.1371/journal.pone.0063852

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