Effects of Moringa oleifera seed extract on PbNP-Toxicity induced in rats

Abstract

This work was conducted to study the protective role of Moringa oliefera seeds extract (MSE) against the toxic effect of commercial lead nanoparticles (CPbNP) in rats. Twenty eight albino male rats were divided into four groups (seven animals in each one) as follow: (A) control group, (B) 160mg/kg body weight per day (b. wt./d.) of M. oliefera (MSE)seed extract, (C): 5mg CPbNP/kg b. wt./d. and (D): MSE + CPbNP. The experimental period was 45 days. Blood was drawn for protein and enzyme estimation at the end of the period of exposure. The rats were dissected and the liver, kidney, spleen and muscle were isolated for Pb estimation. Results showed that the levels of bioaccumulated lead, serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, malondialdehyde were significantly increased in exposed animals to CPbNP (P < 0.05). In contrast, there was a significant reduction of total proteins, albumin, glutathione, superoxide dismutase enzyme and catalase enzyme activities (P < 0. 05) in comparison to control. But the administration of the CPbNP with MSE, all the above biochemical changes were improved significantly. It is concluded that CPbNP has a significant toxic effect on rats, and that seed extract of M. Oleifera can reduce it.