MiRNA-425-5p enhances lung cancer growth via the PTEN/PI3K/AKT signaling axis

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Background: miRNAs regulate a multitude of cellular processes and their aberrant regulation is linked to human cancer. However, the role of miR-425-5p in lung cancer (LCa) is still largely unclear. Here, we explored the role of miR-425-5p during LCa tumorigenesis. Methods: Cell proliferation was evaluated by cell counting Kit-8 and colony formation assay. Western blot and real-time PCR were accordingly used to detect the relevant proteins, miRNA and gene expression. Luciferase reporter assays were used to illustrate the interaction between miR-425-5p and PTEN. Results: We demonstrate that miR-425-5p is overexpressed in LCa tissue and enhances the proliferative and colony formation capacity of the LCa cell lines A549 and NCI-H1299. Through predictive binding assays, PTEN was identified as a direct gene target and its exogenous expression inhibited the pro-cancer effects of miR-425-5p. Through its ability to down-regulate PTEN, miR-425-5p activated the PI3K/AKT axis. Conclusion: We conclude that miR-425-5p promotes LCa tumorigenesis through PTEN/PI3K/AKT signaling.




Zhou, J. S., Yang, Z. S., Cheng, S. Y., Yu, J. H., Huang, C. J., & Feng, Q. (2020). MiRNA-425-5p enhances lung cancer growth via the PTEN/PI3K/AKT signaling axis. BMC Pulmonary Medicine, 20(1). https://doi.org/10.1186/s12890-020-01261-0

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