In Vitro Evaluation of Ozone Activity on Recent Clinically Isolated Bacterial Strains

Abstract

This study aims to evaluate the ozone bactericidal activity in different suspension media (saline, broth and whole blood) at different exposure times. Methicillin-resistant Staphylococcus aureus, Enterococcus faecalis, ESBL-positive Escherichia coli, MDR Pseudomonas aeruginosa were suspended in different media. We used a bacterial concentration of 0.2 MF for all experiments, as this concentration is consistent with the results of septic shock blood experiments. We performed ozone insufflations in a “sealed environment”. The total number of insufflations for each experiment ranged from one to four.