Analysis antifungal compounds produced by endophytic bacteria from oil plant using Bioautography

Abstract

This study was undertaken to evaluate the antifungal activities of the bioactive compounds poduced by endophytic bacteria isolated from oil palm plant using TLC-Bioautography. Antifungal compounds used in this study have been extracted from potential endophytic bacteria of previous studies using ethyl acetate solvent. The antifungal compounds was tested for its activity against the pathogenic fungus Fusarium oxysporum f.sp. by using TLC-Bioautography method. The results showed that there are 3 spots on the TLC plate for samples of B11 antifungal compound with an incubation time of 54 hours and B11 with an incubation time of 24 hours. Observation of bioautographic antifungal activity test showed the presence of inhibition zones with the largest average percentage given by B11 with an incubation time of 24 hours is 11.59%. The inhibition zone provided B11 with an incubation time of 54 hours is only 11.08%.