Determinación y cuantificación de glucósidos de esteviol en las hojas de Stevia comercial (Stevia rebaudiana Bertoni) mediante cromatografía de líquido de alta resolución HPLC

Abstract

Stevia rebaudiana Bertoni (Stevia, ka’â he’ê) is a plant native to our country (Paraguay) with much growth in its production in different countries of the region and the world. This vegetable contains glycosides that are responsible for providing the sweet taste to this plant that was discovered in 1931, they are called steviol glycosides that are of the diterpenoid type and identified as stevioside, steviolbioside, rebaudioside A, B, C, D, E and F and dulcoside. These are composed in the days of the plant in variable percentages depending on the species, growth conditions and agronomic techniques, reaching up to 20% of its composition. In thisresearch work, the determination and quantification of steviol glycosides in commercial warehouses of Stevia (Stevia rebaudiana Bertoni) was carried out, the extraction of plant analytes was carried out by solid-liquid extraction using a soxhlet reflux equipment and was quantified by the HPLC high resolution liquid chromatography technique, giving the following results: Stevioldioside 0.16%, Stevioside 7.35%, Rebaudioside A 14.2%, Rebaudioside B 0.23%, Rebaudioside C 1.59%, Rebaudioside D 0.86%, Dulcoside A ND (Not detectable). The analytical extraction technique and the analytical method used HPLC have shown good performance and efficiency, which could be applied to the quantitative analysis of these most representative glycosides in Stevia reserves of a commercial product.