Protein sorting by lipid phase-like domains supports emergent signaling function in b lymphocyte plasma membranes

0Citations
Citations of this article
127Readers
Mendeley users who have this article in their library.

Abstract

Diverse cellular signaling events, including B cell receptor (BCR) activation, are hypothesized to be facilitated by domains enriched in specific plasma membrane lipids and proteins that resemble liquid-ordered phase-separated domains in model membranes. This concept remains controversial and lacks direct experimental support in intact cells. Here, we visualize ordered and disordered domains in mouse B lymphoma cell membranes using super-resolution fluorescence localization microscopy, demonstrate that clustered BCR resides within ordered phase-like domains capable of sorting key regulators of BCR activation, and present a minimal, predictive model where clustering receptors leads to their collective activation by stabilizing an extended ordered domain. These results provide evidence for the role of membrane domains in BCR signaling and a plausible mechanism of BCR activation via receptor clustering that could be generalized to other signaling pathways. Overall, these studies demonstrate that lipid mediated forces can bias biochemical networks in ways that broadly impact signal transduction.

Cite

CITATION STYLE

APA

Stone, M. B., Shelby, S. A., Nńñez, M. F., Wisser, K., & Veatch, S. L. (2017). Protein sorting by lipid phase-like domains supports emergent signaling function in b lymphocyte plasma membranes. ELife, 6. https://doi.org/10.7554/eLife.19891

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free