GREEN SYNTHESIS AND BIOCHEMICAL PROPERTIES OF PROPOLIS BASED SILVER NANOPARTICLES

Abstract

Propolis is a substance collected by honey bees from different parts of plants. Honey bees store it in their hives in order to defend against different threats. Propolis is a bee-product rich in plant waxes, esters, fatty acids, volatile components and phenolic compounds. It's rich phenolic content makes propolis a potential reducer for Ag+to Ag. In this study, propolis-based silver nanoparticles were obtained using the green synthesis technique. It was determined that the obtained silver nanoparticles had a maximum absorbance at 425 nm and their sizes ranged from 67 to 75 nm. When the FT-IR data of propolis extract is examined, the band at 3200 cm-1represents functional - OH groups, the band at 2919-2850 cm-1represents C-H stretching, the band at 1634 cm-1represents C=C, C=0 or NH stretching, the band at 1508 cm-1represents aromatic C=C stretching and the band at 1451 cm-1originate from the C-H stretching of CH3, CH2, flavonoids and aromatic rings. Total phenolic content of propolis extract and silver nanoparticles was determined as 176.42±0.18 and 122.63±0.23 mg GAE/mL, respectively. IC50 value of P-AgNPs for α-amylase and α-glycosidase enzyme inhibition was defined as 47.08 ± 0.002 and 52.18 ± 0.001 μg/mL, respectively. Inhibition of α-Amylase and α-glycosidase is still a valid approach in the treatment of diabetes. The high inhibition effect of the obtained nanoparticles on the related enzymes shows that they have diabetes treatment potential. In addition, showing that cheap and abundant nanoparticles can be obtained by using propolis, this study may contribute to the development of new products containing nanoparticles that can be used in apitherapy applications.