Gradient Distribution Patterns of Rhizosphere Bacteria Associated with the Coastal Reclamation

Abstract

The main aim of the study was to assess the gradient distribution and composition diversity of microbial taxa using a spatial scale with physicochemical heterogeneity between reclamation area and non-reclamation area. The grid method was utilized to set the sample belt and sample square. The Illumina MiSeq platform was used to study the gradient distribution of rhizosphere bacteria in reclamation and non-reclamation areas. Operational taxonomic units were used to measure bacterial relative abundance. The analysis results showed salt concentration can effect on differences in bacterial relative abundance and composition diversity due to a variable in the rhizosphere soil. Bacterial relative abundance in reclamation area was significantly lower than that in non-reclamation area. The bacterial community composition showed a statistically significant to spatial variability. Some bacterial phyla exit in the whole study area, out of accordance with salinity control. It means that the composition of bacterial community was varied for the environment disturbance, but it was not unique correlated to environment factor. Anaerolinea genus has significantly positive correlation with salinity. It indicated that Anaerolinea genus can provide an indicator to response the environment change.