Analysis of bioactive compounds of caulerpa recemosa, sargassum sp. And gracillaria verrucosa using different solvents

Abstract

This study aimed to analyze the bioactive compounds of Caulerpa recemosa, Sargassum sp., and Gracillaria verrucosa from Makssar Strait using three different solvents i.e. acetone, ethanol, and methanol. Samples of the seaweed C. recemosa collected from marine waters of Takalar regency, while Sargassum sp. and G. verrucosa were collected from marine waters of Pangkep regency which is part of the Makassar Strait. Seaweed crude extract qualitatively analyzed using Thin Layer Chromatography (TLC). The treatment applied in this studi is Sargassum sp. with acetone solvent, Sargassum sp. with ethanol solvent, Sargassum sp. with methanol solvent, C. recemosa with acetone solvent, C. recemosa with ethanol solvent, C. recemosa with methanol solvent, G. verrucosa with acetone solvent, G. verrucosa with ethanol solvent, and G. verrucosa with methanol solvent. Parameters measure were the total extract yield, total phenolic content and phytochemical compound of seaweed crude extrac. The result showed that the content of bioactive compounds crude extract of seaweed is affected by the species of seaweed and solvents used in extraction process. The crude extract of C. recemosa produces better bioactive compounds than the crude extract of G. verrucosa and Sargassum sp. as shown by the yield and total phenolic content of crude extract. The best solvent used in extracting bioactive compounds of C. recemosa is methanol. Identified phytochemical compounds from the crude extract of C. recemosa, are flavonoids, terpenoids, alkaloids, and phenols.