PEMBUATAN BIOETANOL DARI EMPULUR SAGU (Metroxylon spp.) DENGAN MENGGUNAKAN ENZIM

Abstract

Sago , an endemic plant of Indonesia, is potential for bio-ethanol base material. Bioethanol can be producted from sago core, starch as well as fiber by utilizing certain enzymes. This research was conducted to determine the effectivenees of alpha amylase and gluco-amylase enzymes for bio-ethanol production. The process employed in this research include delignification using alpha amylase enzyme, saccharification using gluco amylase enzyme, and 3-day fermentation at temperature 28 C the process concluded with distillation at 78-100 C. Laboratory results showed that producing ethanol from sago strach with a combination of 0.18 mL/liter alpha amylase and 0.48 ml/liter gluco amylase gives the highest percentage of bio-ethanol, namely 65%. The combination of 0.13 mL/liter alpha amylase, 0.11 mL/liter gluco amylase and 0.33g/liter yeast on sago core only produces 44.84% ethanol using the same combination on sago fiber gives as low as 8.26% ethanol. At the small scale, production using strach, core and fiber gives 24%, 11% and 4% ethanol respectively.