Transcript and protein level analyses of the interactions among PhoB, PhoR, PhoU and CreC in response to phosphate starvation in Escherichia coli

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Abstract

The responses to phosphate starvation by PhoR-PhoB two-component regulatory system are also affected by other regulatory systems in Escherichia coli. The interactions among PhoB, PhoR, PhoU and CreC were investigated at transcript and protein levels using real-time PCR and fluorescence resonance energy transfer analyses. CreC showed an interaction with PhoB, before an interaction between PhoB and PhoR, suggesting that this acts as a constant sensor for the early response to phosphate starvation. PhoU was found to interact with neither PhoB nor PhoR, while this acted as a phosphate starvation-inducible protein. These results showed the detailed time- and phosphate concentration-dependent interactions among PhoB, PhoR, PhoU and CreC in response to phosphate starvation in E. coli. © 2007 Federation of European Microbiological Societies.

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APA

Baek, J. H., Kang, Y. J., & Lee, S. Y. (2007). Transcript and protein level analyses of the interactions among PhoB, PhoR, PhoU and CreC in response to phosphate starvation in Escherichia coli. FEMS Microbiology Letters, 277(2), 254–259. https://doi.org/10.1111/j.1574-6968.2007.00965.x

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