Determination of buffalo and pig “Rambak” crackers using ftir spectroscopy and chemometrics

Abstract

This study aimed to identify the type of “rambak” (cracker) by comparing rambak made from buffalo skin and that made from pig skin using fourier transformed infrared spectroscopy (FTIR) method. Samples of lipid obtained during Soxhlet extraction from buffalo skin, pig skin, rambak from buffalo skin and rambak from pig skin was analyzed. The lipid was scanned using FTIR spectrophotometer aided with chemometrics of Partial Least Square (PLS) and Principle Component Analysis (PCA). After optimization procedure, wave number of 1200-1000 cmG1 was selected for analysis. The results showed that the relationship between the predicted value to the true value of pig skin in rambak has coefficient of determination (R2) of 0.96, root mean square of calibration (RMSEC) of 2.56 and Root Mean Square Error of Prediction (RMSEP) of 1.10. The PCA models successfully classify types of buffalo skin, pig skin and commercial rambak. The PLS calibration model and PCA can be used to classification and quantification of the various types of used skin lipid.