The Mold Causing Agent of Rotten Snake Fruit (Salacca zalacca (gaertn.) from Traditional Fruit Markets

Abstract

The Rotten fruits cause of great losses. The molds and other contaminant microbes are the causing agent of rotten fruits. Snake fruit (Salacca zalacca (Gaernth) rotten fungi were found in the garden, market or during transportation to the market. The purpose of the experiment is to study the molds in rotten fruits at the three traditional fruit markets, Bogor, Depok and Jakarta. The mold from 122 fleshy up rotten snake fruits were isolated and identified. Colonies were grown for 3-7 days, at 25-30 °C on Potato Dextrose Agar (PDA) using direct isolation methods. Growth rate were observed every day. Molecular techniques were applied to confirm species identification. Morphology characteristics and DNA sequencing results showed that the isolates were Thielaviopsis paradoxa, (98.3%) and Thielaviopsis ethacetica (1.7%). The molds found in snake fruit from the garden and from the market were similar. Thielaviopsis paradoxa, is the main causes of snake fruit rotten agent in the fruit traditional markets and the initial occurrence of pollution is in the garden.