Detection of B. anthracis spores and vegetative cells with the same monoclonal antibodies

25Citations
Citations of this article
19Readers
Mendeley users who have this article in their library.

Abstract

Bacillus anthracis, the causative agent of anthrax disease, could be used as a biothreat reagent. It is vital to develop a rapid, convenient method to detect B. anthracis. In the current study, three high affinity and specificity monoclonal antibodies (mAbs, designated 8G3, 10C6 and 12F6) have been obtained using fully washed B. anthracis spores as an immunogen. These mAbs, confirmed to direct against EA1 protein, can recognize the surface of B. anthracis spores and intact vegetative cells with high affinity and species-specificity. EA1 has been well known as a major S-layer component of B. anthracis vegetative cells, and it also persistently exists in the spore preparations and bind tightly to the spore surfaces even after rigorous washing. Therefore, these mAbs can be used to build a new and rapid immunoassay for detection of both life forms of B. anthracis, either vegetative cells or spores. © 2009 Wang et al.

Cite

CITATION STYLE

APA

Wang, D. B., Yang, R., Zhang, Z. P., Bi, L. J., You, X. Y., Wei, H. P., … Zhang, X. E. (2009). Detection of B. anthracis spores and vegetative cells with the same monoclonal antibodies. PLoS ONE, 4(11). https://doi.org/10.1371/journal.pone.0007810

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free