Cell discrimination by multiangle light scattering

Abstract

Measurement of the light scattered by biologic cells as a function of scattering angle provides information which can be correlated with cell type. Two flow systems that provide multiangle scattering data from cells have been constructed and tested. The first utilizes two narrow aperture detectors positioned at different angles; the second utilizes the motion of the cell to generate complete scatter patterns of individual cells over a 67° range of scattering angle.