UV determination of epinephrine, uric acid, and acetaminophen in pharmaceutical formulations and some human body fluids using multivariate calibration

Abstract

In this work, a spectrophotometric methodology was applied in order to determine epinephrine (EP), uric acid (UA), and acetaminophen (AC) in pharmaceutical formulations and spiked human serum, plasma, and urine by using a multivariate approach. Multivariate calibration methods such as partial least squares (PLS) methods and its derivates were used to obtain a model for simultaneous determination of EP, UA and AC with good figures of merit and mixture design was in the range of 1.8-35.3, 1.7-16.8, and 1.5-12.1 μg mL-1. The 2nd derivate PLS showed recoveries of 95.3-103.3, 93.3-104.0, and 94.0-105.5 μg mL-1 for EP, UA, and AC, respectively.