Analysis of novel soybean sprout allergens that cause food-induced anaphylaxis

Abstract

Several soybean- and soy product-based allergens have been reported. However, a full analysis of soybean sprout allergens remains to be conducted. Soybean sprout allergyis related to soy intolerance. Here we report a case of anaphylaxis induced by soybean sprouts in a 49-year-old woman. We performed a skin prick test, allergen-specific IgE identification using microarray assay (ImmunoCAP®-ISAC), SDS-PAGE, and immunoblotting for allergen analysis. The ImmunoCAP®-ISAC microarray assay detected Gly m 4 in the soybean sprout. However, immunoblotting revealed two 100-kDa protein bands that did not correspond to Gly m 4 (17 kDa). Therefore, we propose that this patient was not only sensitized to the Gly m 4 allergen, but also to the novel 100-kDa protein.