Effect of linoleic acid supplementation on triglyceride content and gene expression in milk fat synthesis in two- and three-dimensional cultured bovine mammary epithelial cells

Abstract

In this study, the effect of 0, 40, and 80 μmol/L of LA on triglyceride (TG) content was evaluated, as well as the mRNA expression of genes involved in milk fat synthesis in bovine mammary epithelial cells (BMECs) which were cultured in two-dimensional (2D) and three-dimensional (3D) models. TG content was measured with a TG determination kit, and the expression of genes was measured with real-time quantitative polymerase chain reactions (RT-qPCR). Adding 80 μmol/L of LA significantly increased intracellular TG content in both 2D and 3D cultured BMECs (p