Genetic Diversity as Assessed by Agronomical Traits and Molecular Markers in Local Land Races of Green gram ( Vigna radiata L.) Under Rice Fallow Condition

Abstract

Twenty five greengram genotypes were analysed for assessment of genetic diversity through study of agronomical traits and molecular markers. Multivariate analysis of divergence among 25 green gram genotypes for 12 agronomical traits revealed considerable genetic diversity and led to their grouping into four clusters. Reaction of 10 days old plants on exposure to cold contributed maximum, accounting 28.5% of total divergence. A set of 44 SSR markers derived from adzuki bean and greengram was used. Nineteen SSR markers showed amplification and out of which 17 showed polymorphism with an average of 1.63 polymorphic alleles per primer pair. The dendogram constructed based on SSR markers grouped the 25 greengram genotypes into six main clusters. The Jaccard’s similarity coefficient ranged from 0.42 to 0.94. The clustering pattern based on SSR polymorphic data showed consistency with that of multivariate analysis with little deviation. Keywords