Abstract
Rat autotaxin has been cloned, expressed, purified to homogeneity and crystallized via hanging-drop vapour diffusion using PEG 3350 as precipitant and ammonium iodide and sodium thiocyanate as salts. The crystals diffracted to a maximum resolution of 2.05 Å and belonged to space group P1, with unit-cell parameters a = 53.8, b = 63.3, c = 70.5 Å, = 98.8, Β = 106.2, = 99.8°. Preliminary X-ray diffraction analysis indicated the presence of one molecule per asymmetric unit, with a solvent content of 47%. © 2010 International Union of Crystallography All rights reserved.
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Day, J. E., Hall, T., Pegg, L. E., Benson, T. E., Hausmann, J., & Kamtekar, S. (2010). Crystallization and preliminary X-ray diffraction analysis of rat autotaxin. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 66(9), 1127–1129. https://doi.org/10.1107/S1744309110030228
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