Alkaloids piperine in dichloromethane fraction of red galangal rizhome (Alpinia purpurata)

Abstract

Red galangal rizhome (Alpiniapurpurata) was extracted with dichloromethane using maceration methods. The crude extract was fractionated using vacuum column chromatography with a mixture of ethyl acetate:n-hexane (2:3) to give two fractions (F1 and F2). Fraction 2 was fed into the silica gel of column chromatography tailed by recrystallization to produce crystalline (17 mg). The purity was tested by using melting point apparatus and thin-layer chromatography method for any solvent. Their structures were established by spectroscopic methods (UV-Vis, FTIR, 1H-NMR and 13C-NMR). Result of analysis gave melting point of crystals around 130°C. The UV-Vis spectrometer obtained maximum wavelength 344 nm. FTIR analysis showed C=O carbonyl, C-O ether, C-N, and C=C aromatic. Analysis by 1H-NMR spectrometer showed that bioactive compounds have a 19 protons. Analysis by 13C-NMR spectrometer showed that bioactive compounds have a 17 carbon atoms. The results of identification showed that bioactive compounds in dichloromethane fraction of red galangal rhizome was a compound piperine alkaloids types with a chemical formula of C17H19O3N.