Dna break-induced sumoylation is enabled by collaboration between a sumo ligase and the ssdnabinding complex rpa

Abstract

Upon genome damage, large-scale protein sumoylation occurs fromyeast to humans to promoteDNArepair. Currently, the underlying mechanism is largely unknown. Here we show that, upon DNA break induction, the budding yeast SUMO ligase Siz2 collaborates with the ssDNA-binding complex RPA (replication protein A) to induce the sumoylation of recombination factors and confer damage resistance. Both RPA and nuclease-generated ssDNA promote Siz2-mediated sumoylation. Mechanistically, the conserved Siz2 interaction with RPA enables Siz2 localization to damage sites. These findings provide a molecular basis for recruitingSUMOligases to the vicinity of their substrates to induce sumoylation upon DNA damage.