Evaluation of in vitro sucrase inhibitory and non-enzymatic glycation properties of ocimum tenuiflorum l. Leaves

Abstract

Different concentrations of aqueous and ethanolic extracts of Ocimum tenuiflorum L. leaves were evaluated for their sucrase inhibition and non-enzymatic glycation inhibitory effects. The results revealed that aqueous extracts, particularly the one from oven-dried leaves had significant inhibition potential for all three assays. The IC50 values for sucrase inhibition assay, BSA-glucose assay, and BSA-fructose assay were, 370.48±2.14 µg/ml, 188.19±2.52 µg/ml, and 166.16±1.99 µg/ml, respectively and the highest inhibition found in1250 µg/ml were, 91.88±0.16%, 118.46±0.27%, and 104.15±0.21%, respectively. Other than that, the aqueous extract of fresh leaves and 50% ethanol extract of oven dried leaves also showed good inhibition activity. Though, the results presented the highest inhibition activity for BSA-glucose assay. The leaves also attained 50% inhibition with the lowest concentration for BSA-fructose assay. The overall result of O. tenuiflorum leaves suggested that it can be used for treating diabetes mellitus and the complications associated with this.