Chemopreventive Activity of Kola (Cola Accuminata) Seed Ethanol Extract in Mice Induced by Cyclophosphamide

Abstract

Cola accuminata or kola is one of the plants that have activity as anticancer. Previous research has shown that the content of hexane extract from Cola accuminata seeds can kill 100% of breast cancer and prostate cancer. The objective of this study was to demonstrate the presence of chemopreventive activity of ethanol extract of cola seeds in cyclophosphamid-induced test animals. The experiment was conducted using experimental method using healthy mice. The kola seeds were extracted macerated for 48 hours with ethanol solvent. On the first day, the test animal induced by cyclophosphamide with a dose of 50 mg/kgBW intraperitoneal. Ethanol extracts of kola seeds were administered for test animals at doses of 200.400 and 800 mg/kgBW for 2 days then animal was killed and dissected, blood drawn for hematocrit and femoral bone examination for micronucleus examination. Based on result of research known ethanol extract of kola bean able to increase hematocrit value but not statistically significant and on micronucleus observations showed a statistically significant decrease in the amount of micronucleus (P<0,05) with the best activity seen at doses of 800 mg/kgBW. The results concluded that the extract ethanol kola seeds have chemopreventive activity at dose 800 mg/kgBW.