Use of kisser glycerol gelatin to prepare microscope slides of phlebotomine sandflies (Diptera: Psychodidae)

Abstract

Introduction: Appropriate preservation of specimens is important for taxonomic identification. In sandfly research, various methods have been used for slide preparation; however, high cost, low commercial availability, and associated hazards make their use impossible in some studies. Therefore, the efficacy of Kisser glycerol gelatin for sandfly slide preparation was tested. Methods: Kisser glycerol gelatin, as a substitute for Canada balsam and Berlese’s fluid, was used for mounting sandflies. Results: Forty-two mounted specimens were created and maintained even after 14 months. Conclusions: Use of Kisser glycerol gelatin is simple and efficient for preparing microscope slides of sandflies.