The effect of Curcuma zedoaria (Berg) Rosc. plant rhizome extracts on proliferation and viability of myeloid and fibroblas cancer cells

Abstract

This research aimed to look into the effect of extracts from Curcuma zedoaria (Berg) Rosc. plant rhizomes on the proliferation of K-562's myeloid cancer and WeHi-164's fibroblast tumor cells. Two types of extracts, comprising water-media and ethanol-media extracts were prepared; and then both tested at varying concentrations (0, 0.1, 1.0, 10.0, and 100.0 (μg/ml). Proliferation activities were performed on all extract concentrations, either water or ethanol media. Observation focused on morphology-anatomy characteristics of cancer cells. Both extract types (water and ethanol media) inflicted anti-proliferation actions on cancer cells. The lowering/decreasing intensity of the cell proliferation as affected by plant extracts was significant at high extract concentration (100.9 (μg/ml), with water as well as ethanol media; but less effective at low concentrations (0.1-10.0 (μg/ml). Anti-proliferation activities by plant extracts at low concentration altered morphology, shapes, and sizes of K-562's and WeHi-164's cells, but their cell wall remained intact (unchanged). The use of plant extracts with ethanol media at high concentration altered the shapes/morphology, sizes of K-562's myeloid cells, fibroblast cells enlarging, cell wall fractured, and the cells fragmented. Plant extracts with ethanol media afforded stronger anti-proliferation activities than those with water media.