DISCRIMINANT ANALYSIS OF PURIFICATION ON TURMERIC (CURCUMA LONGA LINN) SAMPLES BASED ON PHYTO-PHARMACOGNOSTICAL AND MULTIVARIATE CHEMOMETRIC TECHNIQUE

Abstract

Objective: To establish an acceptable identification system of various purification effects in context classical based different media on turmeric samples and relates its altering pattern in favor of phyto-pharmacognostical, image processing and multivariate chemometric analysis.Methods: Authenticated turmeric samples purified through different processes by using different media such as cow’s urine, panchapllava (five different plants tender leaves), the inflorescence of alambusha (Sphaeranthus indicus, Linn) decoction, water and buttermilk. Resultant samples dried, pulverized and undertaken powder microscopy, image processing, physicochemical and chromatographic fingerprinting (HPTLC). The multivariate chemometric analysis, principal component analysis (PCA) analyzed with help of Unscrambler and image processing in Matlab software.Results: The addition of characters of medias drug with turmeric powders like the crystal of gomutra, pollen grain and starch grain of Alambusha, epidermis, fibre, the crystal of panchapllava. Identify different perceivable colors in variously processed turmeric by analyzing the Lab color space through the Image segmentation. PC1 and PC2 explained (90 + 9) % total variance in score plot of respective purify turmeric samples shown clear grouping in relation to the physicochemical constant. Quantification of curcumin in various treated turmeric samples displayed variation due to additive effect in high-performance thin layer chromatographic profile.Conclusion: This study proved that purification in ayurveda not only refers to the elimination of toxins and unwanted particles but also the transformation in the properties in the primary substance rendering it safe as well as many desired qualities are imbibed in it.