Optimizing the immobilization of lipase enzyme (Aspergillus oryzae) in the silica and silica-cellulose matrix by adsorption method

Abstract

Silica and silica-cellulose were safe biomaterials used as the matrix for immobilized enzyme or any bigger molecule. Some enzymes play role in some processes to biofuel production. In this study, the enzyme used was the lipase enzyme, to test the ability of the matrix as carrier. This research aimed to know the ability to immobilize lipase enzyme using adsorption method. Spectroscopy methods were involved. The research was divided into 4 steps (1) preparation of silica and silica-cellulose matrices, (2) characterization of silica and silica-cellulose, (3) immobilization of lipase enzyme on the silica and silica-cellulose which included activity test: optimum temperature, optimum pH, and optimum stirring speed, and (4) effectiveness of immobilized lipase enzyme on the silica and silica-cellulose. The result showed silica porous material as a matrix was more effective than silica-cellulose as matrix for lipase enzyme.