A transposable approach to RNA-seq from total RNA

Abstract

The rapid progress of RNA-seq technology requires the development of simple, versatile tools that competently reflect RNA levels in the cell. We have developed the TotalScript™ RNA-Seq Kit, a new, transposome-based directional library prep method suitable for a variety of RNA sample types and a range of input. TotalScript libraries exhibit high complexity, ~97–99% directionality and low rRNA contamination. The method is scalable and robust, allowing input amounts of ~100 pg of poly(A)+ RNA or ~5 ng of total RNA