Practical Staining Method of Well-Unstained Haematological Specimens

Abstract

In hematology, May-Grunwald-Giemsa (MGG) staining is used to evaluate bone marrow aspirate as well as blood smear. This is an easy method in order to avoid the damage of the cells; good qualities of the stain and its directives, as well as the duration of the procedure are very important. The smears need to be dried quickly after the procedure. For a successful smear needs to be thin and while drying it up, hot blowing can be used. In case bone aspirate or blood smear are not well stained by MGG, the aspiration has to be re-executed or the biopsy results should be awaited. That procedure takes approximately 15days that is needed to get the biopsy result and a long time that leads to material and motivational loss. In our clinic, we use a practical stain method for pre-diagnosis in such cases. Two cases are presented in this study inform you about that method that yielded good results on a first trial.