Phenotypic differences between cord blood and adult peripheral blood

Abstract

Background and Objectives: Neonates are more susceptible to infections than adults, due to a less mature immune system. The objective of this study was to compare the immunophenotypes of cord blood (CB) and adult peripheral blood (APB) and to establish whether or not there are immunophenotypical differences. Methods: CB and APB were collected into CPD anticoagulant. Whole blood was stained with fluorochrome conjugated antibodies, cells were fixed, red cells were lysed, and samples were run in a FACS-Canto flow cytometer. Results: Plots of SSC vs. CD45 showed two lymphocyte-like populations in CB with the same low SSC characteristics while there was only one low SSC lymphoid population in APB. The CD45 dim population included the majority of CD34 + cells, but it also included T, B, NKT, and NK cells. The percentages of CD3 +, CD3 +CD4 +, CD3 +CD8 +, CD19 +, CD3 +CD56 +, and CD3 -CD56 + in the CD45 high gate of CB were similar to the percentages obtained for APB. Meanwhile, in the CD45 dim gate, the percentages were either lower (CD3 +, CD3 +CD4 +, CD3 +CD8 +) or higher (CD19 +, CD3 +CD56 +, CD3 +CD56 +) than in the CD45 high gate. Conclusions: CB presents two lymphoid populations defined by different levels of expression of the phosphatase CD45; the CD45 dim subsets likely represent cells with elevated proliferative activity possibly due to the differential expression of CD45 but still not yet immunologically mature with regard to immunophenotype and function. © 2008 Clinical Cytometry Society.