A synthetic kiss of death: Expressed protein ligation of a ubiquitin-peptide conjugate

Abstract

Cys no longer required. A recent report from the Muir group describes access to monoubiquitylated histone peptides by expressed protein ligation (EPL). This convenient strategy employs a photolabile thiol auxiliary that allows site-specific ubiquitylation of a Lys side chain and removes need for a Cys residue at the ligation site. (Figure Presented). © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.