Growth of the phototrophic purple sulfur bacterium Thiocapsaroseopersicina under oxic/anoxic regimens in the light

Abstract

Abstract The phototrophic purple sulfur bacterium Thiocapsa roseopersicina was grown in sulfide‐limited continuous cultures exposed to oxic/anoxic regimens in continuous light. Synthesis of bacteriochlorophyll a (BChl a) did not occur during the oxic periods, but started immediately upon the creation of anoxic conditions. In contrast, protein synthesis continued during both oxic and anoxic periods. Consequently, the specific content of BChla fluctuated. Despite the presence of oxygen and the fluctuating BChla content, growth occurred predominantly in a phototrophic mode and respiration was virtually zero. BChla synthesis continued at high rates during anoxic periods, thus compensating for the lack of synthesis during oxic periods. When cultivated under regimens with oxic periods shorter than 12 h the highest specific BCha content was 27 μg?mg− protein. In contrast, when cultivated under regimens with oxic periods longer than 12 h the specific BChla content was always lower than 27μg?mg− length of the oxic periods. During the anoxic periods, BChla synthesis occurred at the maximal velocity of 1.2 μg?mg−1 protein?h−, but the length of the anoxic periods was not sufficient to allow the BChla content to reach the maximum level. Cultivation under continuously oxic conditions eventually resulted in pigmentless cells growing chemolithotrophically. The BChla synthesizing ability was not lost during prolonged exposure to oxygen. It was concluded that T. roseopersicina is very well adapted to oxic/anoxic cycles. Copyright © 1990, Wiley Blackwell. All rights reserved