Use of bacteriophages as biological control agents in horticulture

Abstract

Bacterial diseases in horticultural settings or infestation of fresh produce with human pathogenic bacteria can constitute a serious public health risk. To control horticultural bacterial diseases, chemical control strategies have traditionally been used, such as the application of bactericides and copper-based products, which resulted in development of resistance in bacteria against these agents. Moreover, the use of such chemical preventative measures on fresh produce can detrimentally affect human, animal and ecosystem health. Bacteriophages have been used to control pathogenic bacteria since the 1920 sdueto their specificity against host bacteria, as well as their ability to survive and infect their host without detrimental effects to the surrounding environments. As a result, their targeted host specific applications in horticultural settings canbe of interest to growers as well as to the consumers. In this laboratory report, the efficacy of a bacteriophage cocktail when applied to fresh herbs inoculated with Escherichia coli was determined. Significant (P ≤ 0.001) reductions in E. coli colony forming units were observed in phage treated herb samples compared to counts in the control. These findings suggest that bacteriophage present as an alternative biocontrol for E. coli in horticulture.