Comparison between molecular and classical techniques for identification of Mycoplasma species isolated from mastitic ruminants

Abstract

165 Cows and 19 buffaloes were examined to detect the Mycoplasma mastitis, the result revealed that 114 (69.59{%}) and 6 (31,57{%}) were clinically mastitic cows and buffaloes respectively while 51 (30.9{%}) and 13 (68.42{%}) were apparently healthy cows and buffaloes respectively. On examining the apparently healthy cows and buffaloes, the result were 67 (32.84{%}) and 18 (34.61{%}) from subclinically mastitic cows and buffaloes respectively while 137(67.15{%}) and 34 (65.38{%}) fro apparently completely healthy. Mycoplasma were isolated in percentages of 8.9{%}, 5.5{%} from subclinically mastitic cow and buffaloes respectively and in percentages of 12.97{%}, 12.5{%} from clinically mastitic cows and buffaloes respectively. M. bovis was isolated from 8 (32{%}) and M. bovigenitalium was in percentage of 7 (28{%}) and the unidentified Mycoplasma was 10 (40{%}). Isolation of Mycoplasma from udder tissue in cows and buffaloes were in percentage of 2 (28.5{%}) in cows while no Mycoplasma isolates were obtained from buffaloes udder tissues. Application of PCR technique on these isolates and some negative samples, these were positive with percentage 100{%}. On the other hand, 192 sheep and 118 goats were examined. We found that in percentage of 82 (42.7{%}) and 43 (36.44) from sheep and goats respectively were clinically mastitic. Isolation of Mycoplasma was in percentage of 11 (13.41{%}) and 17 (39.53{%}) of sheep and goat respectively. Identification of these isolates revealed 8 (29{%}) was M. agalactia isolates and 20 (71{%}) was unidentified Mycoplasma spp. Application of PCR technique on M. agalactia isolates which identified by traditional techniques by use specific primers to M. agalactia revealed negative results but on using the primer specific to M. bovis to the same isolates, it was positive to all isolates 8 (100{%}).