Engineering nanoscale hierarchical morphologies and geometrical shapes for microbial inactivation in aqueous solution

Abstract

Here, we study the effect of hierarchical and one-dimensional (1D) metal oxide nanorods (H-NRs) such as γ-Al2O3, β-MnO2, and ZnO as microbial inhibitors on the antimicrobial efficiency in aqueous solution. These microbial inhibitors are fabricated in a diverse range of nanoscale hierarchical morphologies and geometrical shapes that have effective surface exposure, and well-defined 1D orientation. For instance, γ-Al2O3 H-NRs with 20 nm width and ˂0.5 μm length are grown dominantly in the [400] direction. The wurtzite structures of β–MnO2 H-NRs with 30 nm width and 0.5–1 μm length are preferentially oriented in the [100] direction. Longitudinal H-NRs with a width of 40 nm and length of 1 μm are controlled with ZnO wurtzite structure and grown in [0001] direction. The antimicrobial efficiency of H-NRs was evaluated through experimental assays using a set of microorganisms (Gram-positive Staphylococcus aureus, Bacillus thuriginesis, and Bacillus subtilis) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria. Minimal inhibitory and minimum bactericidal concentrations (MIC and MBC) were determined. These 1D H-NRs exhibited antibacterial activity against all the used strains. The active surface exposure sites of H-NRs play a key role in the strong interaction with the thiol units of vital bacterial enzymes, leading to microbial inactivation. Our finding indicates that the biological effect of the H-NR surface planes on microbial inhibition is decreased in the order of [400]-γ-Al2O3 > [100]-β–MnO2 > [0001]-ZnO geometrics. The lowest key values including MIC (1.146 and 0.250 μg/mL), MBC (1.146, 0.313 μg/mL), and MIC/MFC (0.375 and 0.375 μg/mL) are achieved for [400]-plane γ-Al2O3 surfaces when tested against Gram-positive and -negative bacteria, respectively. Among the three H-NRs, the smallest diameter size and length, the largest surface area, and the active exposure [400] direction of γ-Al2O3 H-NRs could provide the highest microbial inactivation.