Immunohistochemical Demonstration of Calcium-Binding Protein Regucalcin in the Tissues of Rats: The Protein Localizes in Liver and Brain

Abstract

A calcium-binding protein, regucalcin, was isolated from rat liver cytosol. Rabbit-anti-regucalcin antiserum, which was raised against regucalcin conjugated by glutaraldehyde to bovine serum albumin, was appliedtoglutaraldehyde-fixed whole mounts and subsequently visulized using the peroxidase-antiperoxidase methods. Rathepatic regucalcin spleen, lung and smooth muscle(bladder), and appeared only slightly in the kidney and heart. Control experiments that regualcin localizes in the liver and brain of rats. © 1991, The Pharmaceutical Society of Japan. All rights reserved.