Anti-adhesive effect of naturally obtained dicalcium phosphate dihydrate nanoparticles in the rat uterine wound model

Abstract

Objective: In this study, we aimed to compare the anti-adhesive effects of contractubex and dicalcium phosphate dihydrate (DCPD) particles in rats treated with the uterine horn adhesion model. Materials and methods: Newly adult, 60 Wistar albino rats were used as experimental animals. The modified rat uterine horn adhesion model was used to induce intra-abdominal adhesion. Tumor necrosis factor (TNF)-α, interleukin (IL)-1, vascular endothelial growth factor (VEGF) and transforming growth factor (TGF)-β1 were studied for biochemical and immunohistochemical examination. Results: TNF-α decreased in each group, while it decreased more in G2 and G3 than in G1. IL-1β decreased in each group, while it decreased the most in G3. TGF-β1 and VEGF localization was less in the G2 compared to G1, the least TGF-β1 and VEGF immunolocalization was detected in the G3 and G4. For both antibodies, the least localization among all groups belonged to G3. From day 7 to day 21, the highest TGF-β1 immunolocalization was observed in G1, lesser localization in G2 and lowest in G3. Conclusion: DCPD nanoparticles show promise as a clinical antiadhesive agent and should be further evaluated in experimental animal models and human trials.