Antimicrobial and Antibiofilm Activity of Cinnamon Oil on Staphylococcus aureus Isolated from Cattle Milk with Mastitis

Abstract

Background: Dairy sector is a leading industry in our country. Mastitis being most concerned and frightful disease, not only affecting health of animals but also the economy of the livestock owner. Prevailing status of the antimicrobial resistance makes the treatment of mastitis much more difficult. Biofilm formation in mastitis milk could place difficulty in the treatment of animals as it further increases the possibility of antimicrobial resistance. Essential oils generally being less toxic, could be optimal alternatives to conventional antibiotics, most of these essential oils higher MIC values than antibiotics, needed to be given in increased concentration in feed/water. Methods: Different dairy farms were screened for mastitis (164 dairy cattle and buffalo) Staphylococcus aureus isolates were identified and characterized phenotypically by standard biochemical tests. The biofilm formation ability was studied by different methods along with the quantitative analysis and MIC of standard drug cefoperazone. Result: Out of total milk samples 17 samples were identified Staphylococcus aureus positive with Congo red agar (70% positive), Light Microscopy method (52% positive) and Microtiter plate (Crystal Violet) method. (100% sensitivity). The antimicrobial and antibiofilm effect of Cinnamon oil were studied by Microtiter plate method, SEM analysis and confocal techniques. Cinnamon oil gave antibiofilm activity in all the concentration used (1%, 2%, 3%). The MIC of Cefoperazone was in the range of 64 μg/to 16 μg/ml. No significant changes were observed on biofilm production at sub-MIC of cefoperazone.