Maltodextrin-Based Deep Eutectic Solvent as a Chiral Selector for Enantioseparation of Azole Antifungal Drugs in Capillary Electrophoresis

Abstract

Deep eutectic solvents have attracted increasing attention in enantioseparation, but only a few studies focus on the development of deep eutectic solvents as the sole chiral selector. In this work, a one-pot synthetic method was used to prepare a deep eutectic solvent based on a polysaccharide chiral selector (maltodextrin) as the sole chiral selector in capillary electrophoresis. Six azole antifungal drugs were used as model drugs to investigate the chiral resolution ability of the maltodextrin-glycerol. Compared to native maltodextrin, the deep eutectic solvents exhibited the more excellent enantioselectivity towards model drugs. Some key factors affecting separation such as maltodextrin concentration, glycerol concentration, buffer pH and separation voltage were investigated. In addition, ultraviolet spectroscopy method and Statistical Product and Service Solutions analysis were adopted to investigate the enhanced chiral recognition mechanism. It is the first time that polysaccharide-based deep eutectic solvents was developed as a favorable chiral selector in capillary electrophoresis, and this research strategy has paved a new way for developing novel chiral materials.