Alternative Metodology for Gold Nanoparticles Diameter Characterization Using PCA Technique and UV-VIS Spectrophotometry

Abstract

The rapid developments in nanostructured materials and nanotechnology is starting to have a profound impact in many applications of the biomedical areas, including biomolecules, tissue engineering, and detection of biomarkers, cancer diagnosis, cancer therapy, and bioimaging. Nanotechnology applications hinge frequently on the availability in well-characterized size distributions for gold nanoparticles. In this work we implemented a methodology in order to determine gold nanoparticles size by using the UV-Vis spectra and the multivariate analysis with the principal components' analysis method. The sample size of gold nanoparticles used for correlation of this methodology were previously known (5, 10, 15, 20, 30, 40, 50, 60, and 80 nm), they were supplied by Sigma Aldrich and Ted Pella Inc. All GNPs were measurement with a spectrometer Shimadzu UV-VIS 1800 Rayleigh model over the range from 400 to 700 nm. The UV-VIS spectra was compared with the implemented methodology in order to show excellent behavior and similitude in results, which one suggests this is an outstanding usefully tool to determine the gold nanoparticle diameter.