Interferon-γ suppresses cyclooxygenase-2 promoter activity by inhibiting C-Jun and C/EBPβ binding

Abstract

OBJECTIVE - Cyclooxygenase-2 (COX-2) and interferon γ (IFNγ) are overexpressed in vascular inflammatory and atherosclerotic lesions. We postulated that IFNγ suppresses COX-2 expression at the transcriptional level. METHODS AND RESULTS - The effect of IFNγ on COX-2 expression was evaluated in several types of human cells stimulated with phorbol 12-myristate 13-acetate (PMA), interleukin (IL)-1β, or tumor necrosis factor (TNF) α. IFNγ concentration-dependently inhibited COX-2 proteins and promoter activities induced by PMA or cytokines in human fibroblasts and monocytic and endothelial cells. PMA and cytokines stimulate binding of C-Jun, C-Fos, CCAAT/enhancer binding protein β (C/EBPβ), or NF-κB to their respective regulatory elements on COX-2 promoter. IFNγ blocked C-Jun and C/EBPβ but not C-Fos or p50 NF-κB binding as determined by in vitro binding assays and chromatin immunoprecipitation assay. p300 binding to COX-2 promoter was inhibited by IFNγ in a manner comparable to C-Jun and C/EBPβ binding. CONCLUSIONS - IFNγ suppresses proinflammatory mediator-induced COX-2 transcription by selective inhibition of C-Jun and C/EBPβ DNA binding activity and p300 recruitment in human cells. Because IFNγ is coexpressed with COX-2 in vascular lesions, it may play a role in controlling COX-2-mediated inflammatory changes. © 2007 American Heart Association, Inc.